CRISPR/Cas12a is a promising genome editing system for targeting AT-rich genomic regions. Comprehensive genome engineering requires simultaneous targeting of multiple genes at defined locations. Here at Lifeasible, to expand the targeting scope of Cas12a, we screen many Cas12a orthologs that have not been demonstrated in plants, and identify ErCas12a, TsCas12a, BsCas12a, MbCas12a, etc. Among them, Mb2Cas12a stands out with high editing efficiency and tolerance to low temperature. To enable large-scale genome engineering, we developed a Cas12a system that exhibits nearly 100% biallelic editing efficiency with the ability to target as many as 16 sites in rice and many other plants.